|  高原鼠兔低氧诱导因子-1α的初步研究 | 
                    
                     
                      | ------------------------------------------------------------------------------------------ | 
                    
                     
                      | 出 版 社:兽类学报23(3):273-276 | 
                    
                     
                      | 发表时间:2003 | 
                    
                     
                      | 台  站:
                        海北高寒草甸生态系统定位研究站 | 
                    
                     
                      | 作  者:赵同标 赵新全 常智杰 朱姗姗 赵伟 孙平 徐世晓 | 
                    
                     
                      | 点 击 率:5806 | 
                     
                      | ------------------------------------------------------------------------------------------ | 
                    
                     
                      | 关 键 字:关键词:低氧诱导因子-1α;高原鼠兔;RT-PCR;青藏高原 | 
                    
                     
                      | 关 键 字(英文):Keywords:Plateaupika(Ochotonacurzoniae);HIF 1α;RT PCR;Qinghai TibetPlateau | 
                    
                     
                      | 摘  要(英文):Abstract:Hypoxia inducedfactor 1playsakeyroleduringthecellhypoxiatransduction Hypoxiainducedfactor 1α(HIF 1α)isafunctionalsubunitofhypoxia inducedfactor 1 Plateaupika(Ochotonacurzoniae),whichisaQinghai Tibetplateaunativeanimallivedabove3000m,hashighratioofoxygenutilizationtoadapttoplateauhypoxiaenvironments Onefragmentofthecodingre gionofcDNAsequenceofplateaupikaHIF 1αwasobtainedbyRT PCRtechniqueusingadegeneracyPCRprimerbasedonprevi ouslyreportedcDNAsequenceinhuman,cattle,housemouseandNorwayratHIF 1αgene ItwasdirectlyinsertedintothevectorpMD18 T DNAsequencingprovedthatitwashighlyhomologywithhuman(91%),cattle(91%),housemouse(89%)andNor wayrat(89%)HIF 1αgene ThestudyprovidesbasicimportantinformationfortheHIF 1αcDNAwholesequencecloningofpla teaupikaanditsfunctionalstudy | 
                    
                    
                   
                     
                      | ------------------------------------------------------------------------------------------ | 
                     
                      |  |